FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072115

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072115
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5458107
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCTT12050.050.9930421
GCGACTT7550.047.5515141
GCGTATC4950.047.4221951
GCGCCCT10950.046.9731941
GCGCATC4150.045.7501531
GCGTCTA6050.045.0764431
GCGCCTA8600.044.555621
GCGACCA6450.044.4218141
GCGCGTT2100.044.383591
GCGACTG6500.044.080111
CGGTCCT6600.043.9352721
CGGTACT6050.043.935271
GGCCCTA36400.043.8145681
GGCCCAT58300.043.6984251
GCGTACT4600.043.5259361
GCGTACA5650.043.379831
CGGTATG7250.043.3292661
CGGTCTT11200.042.842491
GCGTACC3650.042.5596051
GCGTCTC8850.042.5168571
Produced by FastQC (version 0.11.5)