FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072127

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072127
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34731829
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT188150.059.878911
TCCGATC213400.052.79939710
ACGCTCT218250.051.404783
TTCCGAT220150.050.914129
CCGATCT220950.050.54240811
GACGCTC228900.049.2015882
CGCTCTT231700.047.8698584
CTTCCGA260650.043.069228
TCTTCCG281400.039.9791837
CGATCTA45450.039.2431512
CGATCTT101700.034.53297812
CGATCTC135250.029.84391412
CGATCTG81650.024.21060612
GCTCTTC488300.023.3926645
CTCTTCC736750.015.7709976
GATCTTA98200.09.590878513
GATCTAG68550.09.10915913
GATCTCC210550.08.84801313
GATCTAC77100.07.920016313
GATCTTG200300.07.871227713
Produced by FastQC (version 0.11.5)