FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072128

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072128
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34731829
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGATC180400.048.1525211
CCGATCT191400.045.4578912
TTCCGAT191550.045.33008210
CGTGTGC204850.043.4947051
CTTCCGA222850.039.2241749
CGATCTA42400.035.4757113
TCTTCCG252600.034.712848
CGATCTT90350.030.43270913
GTGTGCT302000.030.0740452
GTGCTCT333500.026.8300884
TGTGCTC357050.025.2343463
CGATCTC127300.024.1739113
CGATCTG75950.020.75863813
GCTCTTC449750.019.9718256
TGCTCTT451250.019.966595
CTCTTCC689250.013.4774437
CGACGCT24800.010.5708491
GATCTTA96800.08.19715114
GATCTAG64050.07.864012214
GATCTCC212500.07.37067714
Produced by FastQC (version 0.11.5)