FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072130

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072130
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2930450
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT4900.032.3877561
TCGCCGG751.6704034E-632.269
TCCGATC7350.029.5714281
AACTCGC1301.9826984E-1029.1923087
CGTATCA7150.028.9510557
CGATCTA2550.027.0588233
GCCGTAT7400.027.0405455
CCGTATC7700.026.88311856
CCGATCT7850.025.9299372
CGCCGTA8200.025.66463554
ACTCGCC1651.1459633E-1025.090918
TCGCCGT9300.023.37096853
GTCGCCG11550.020.3116952
TTGATCG1701.08635504E-720.29411715
ACCGGAG1552.247966E-517.80645228
ATCGTTG1653.8448663E-516.72727418
GGTCGCC14600.016.30479451
CCGGATC1300.00252296215.92307755
GATCGTT1958.991863E-615.92307617
GTATCAT13550.015.78597858
Produced by FastQC (version 0.11.5)