FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072131

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072131
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3219386
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCTA200.00736023151.7538
CGACGCT754.024514E-836.8000031
GACGCTC2252.564775E-1019.9333321
GCCGTCT9000.019.16666669
TGCCGTC12750.018.67058869
TATGCCG22450.018.44097969
ACGCTCT3603.45608E-1115.3333331
GTATGCC33300.015.02252269
CGTCTTC6550.014.74809269
CGCTCTT4051.4551915E-1114.4814822
CGTATGC37150.014.02288169
ATGCCGT17300.013.95953769
CCGTCTT7400.013.05405468
TCTCGTA59450.012.07064769
TCGTATG45000.011.65333368
CTCGTAT52350.011.33524369
CCGAGTA6352.3283064E-1010.3228342
ATCTCGT70950.010.1627968
CCCGAGT8801.8189894E-129.409091
TATCTCG80300.08.97945267
Produced by FastQC (version 0.11.5)