FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072133

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072133
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2969974
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGCTC2300.031.51
ATGCCGT17650.023.06515569
TATGCCG24450.020.88343669
CGACGCT850.004121694220.2941191
ACGCTCT3850.019.7142872
GCCGTCT8650.019.54335269
TGGTCGG950.00783729718.1578967
CCGTCTT7150.017.3706369
TGCCGTC11350.017.02202669
CGCTCTT4550.016.6813183
GTATGCC34550.014.978291568
CCTCGGA1851.02217906E-414.918921
TCGTATG51850.014.83799469
CGTATGC40100.013.59351669
CGTCTTC5400.013.41666769
CTCGTAT62550.013.01678869
TGCGCAA5950.012.75630369
TGCGCTG1900.00210991312.71052629
TCTCGTA69850.011.45883969
ATCTCGT83200.010.65685169
Produced by FastQC (version 0.11.5)