FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16190038
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA72000.078.4446269
TCTTCCG82150.068.577278
CGTGTGC90600.062.5164681
CGACGCT6500.043.569171
GTGCTCT169200.033.7691354
GTGTGCT182450.031.836162
TGTGCTC187700.030.5941523
GCTCTTC192300.030.0119956
TCCGATC60000.021.15319810-14
TGCTCTT279100.020.9103265
CCGATCT64950.020.80391710-14
CTCTTCC328400.017.7901217
TTCCGAT66050.017.62521610-14
CGTTGGA88350.017.342191
ACGGGAT60350.016.0946879
AACGGGA82900.014.7543628
AAACGGG98550.014.6015647
CGATCTA20550.014.2148710-14
GCGTCGG26400.013.932024140-144
GAAACGG105600.013.7653496
Produced by FastQC (version 0.11.5)