FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16042102
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA69250.066.434999
TCTTCCG78000.059.3515668
CGTGTGC87750.054.1746061
CGACGCT6600.047.1413571
GTGCTCT163800.029.2285544
GTGTGCT180600.027.2075652
TGTGCTC185450.026.1305623
GCTCTTC193350.024.4645376
CCGATCT64250.019.6995310-14
TCCGATC59900.019.39933410-14
TGCTCTT270150.017.9893135
CGTTGGA87700.017.2434521
ACGGGAT57250.015.719929
AACGGGA77450.015.7101768
GCGTCGG25000.015.090459140-144
AAACGGG98950.014.6977677
TTCCGAT65500.014.66306210-14
CTCTTCC331800.014.4949227
GAAACGG110150.013.1379426
CGATCTA20250.012.7993410-14
Produced by FastQC (version 0.11.5)