FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12966816
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT95450.0138.407261
GACGCTC105350.0124.9439242
ACGCTCT113300.0115.922823
TTCCGAT112800.0115.5305259
CTTCCGA116950.0111.061718
CGCTCTT119550.0108.660564
TCTTCCG122900.0105.509197
GCTCTTC209100.062.564645
CTCTTCC314150.041.7577446
CCGATCT113200.024.82962810-14
TCCGATC109500.024.69602410-14
CGATCTA25400.019.49122410-14
CGTTGGA69200.016.9253921
CGACGTC5251.9404979E-816.6280331
CGATCTT61600.015.91038910-14
CGATCTG31950.015.4953710-14
CGTGTGC34500.014.7603931
AAACGGG81450.013.4288847
ACGGGAT48150.013.3009029
CGACGCC4955.182284E-513.2268461
Produced by FastQC (version 0.11.5)