FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12966816
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA60850.078.392429
TCTTCCG65150.073.770578
CGTGTGC73950.064.7975161
CGACGCT5750.047.9808921
GTGCTCT139050.035.243254
GTGTGCT147450.033.5832142
TGTGCTC149550.032.672563
GCTCTTC149650.032.506476
TGCTCTT224100.021.8035775
CCGATCT53450.021.24149710-14
TCCGATC50350.020.92027510-14
CTCTTCC263600.019.0242927
TTCCGAT55300.017.4343410-14
CGTTGGA68400.016.452361
ACGGGAT47500.016.0558539
GACGCTC14250.014.6474392
AACGGGA65350.014.4227088
AAACGGG76850.014.2305227
CGACGTC4703.2441534E-513.9026381
CGATCTA16750.013.65960510-14
Produced by FastQC (version 0.11.5)