FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14083456
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT82200.0135.801481
GACGCTC91250.0121.022152
ACGCTCT98700.0112.064513
TTCCGAT103650.0107.167139
CTTCCGA105950.0104.2291958
CGCTCTT108050.0101.076924
TCTTCCG112250.098.446997
GCTCTTC210250.052.869175
CTCTTCC326950.034.1736036
CCGATCT102250.023.67011310-14
TCCGATC96900.023.62486310-14
CGATCTA26800.018.21213310-14
CGTTGGA71600.017.3790441
AAACGGG79550.014.2082277
AACGGGA67950.014.1964698
CGATCTT57700.013.77397210-14
ACGGGAT51000.013.6919899
CGATCTG30750.013.672049510-14
GAAACGG88250.012.80752856
GGAAACG93000.012.1536845
Produced by FastQC (version 0.11.5)