FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14083456
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA54000.067.7306069
TCTTCCG62400.059.189938
CGTGTGC71100.052.917071
CGACGCT5850.050.7095831
GTGTGCT150800.026.171972
GTGCTCT147100.025.8906274
TGTGCTC160400.023.8823053
GCTCTTC162200.023.3480136
CCGATCT51000.019.82019610-14
TCCGATC47050.019.73972510-14
GACGCTC16200.016.9246222
TGCTCTT234250.016.4125755
TTCCGAT50550.015.951889
ACGGGAT51950.015.3834049
CGTTGGA74700.015.3037731
GCGTCGG16600.014.139091140-144
CTCTTCC280250.014.0526017
CGACGTC5205.619657E-613.9142151
AACGGGA70950.013.2933358
AAACGGG84600.013.2760617
Produced by FastQC (version 0.11.5)