FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13462588
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT90100.0138.5371
GACGCTC102550.0121.349442
ACGCTCT108450.0114.879553
TTCCGAT109350.0113.319359
CTTCCGA113650.0109.158498
CGCTCTT117200.0105.078094
TCTTCCG121050.0102.069327
GCTCTTC211600.059.106925
CTCTTCC338800.036.978146
CCGATCT108200.024.6204310-14
TCCGATC104750.024.22226310-14
CGATCTA25700.019.31979410-14
CGTTGGA66250.018.2261731
CGACGTC4501.1836764E-616.1644441
ACGGGAT48350.015.1807579
AAACGGG76900.014.8785467
AACGGGA64850.014.8690958
CGATCTG31800.014.79916210-14
CGATCTT62000.014.53112110-14
GAAACGG83000.014.4786516
Produced by FastQC (version 0.11.5)