FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12685446
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT78150.0136.701781
GACGCTC90400.0117.906512
ACGCTCT96000.0111.292913
TTCCGAT96650.0111.279229
CTTCCGA99650.0107.640168
CGCTCTT102750.0102.993814
TCTTCCG106200.0100.933947
GCTCTTC195450.055.434035
CTCTTCC317350.034.2308586
TCCGATC92000.023.9610-14
CCGATCT97150.023.6383610-14
CGTTGGA60200.017.6621481
CGATCTA24650.017.6396310-14
ACGGGAT43900.015.90642459
AACGGGA59600.014.4944048
AAACGGG72200.014.3579487
CGATCTT59600.013.89060110-14
GAAACGG76650.013.4304626
CGATCTG31850.013.42599410-14
CGACGTC4402.9831775E-413.1510361
Produced by FastQC (version 0.11.5)