FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072148

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072148
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12685446
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA54900.063.7361539
TCTTCCG62100.056.3464558
CGTGTGC69700.050.663331
CGACGCT6200.050.1860961
GTGCTCT129500.027.2410774
TGTGCTC139350.025.939393
GTGTGCT140350.025.7064842
GCTCTTC151150.023.6739226
CCGATCT50750.019.18025410-14
TCCGATC48000.018.44920310-14
CGTTGGA63250.018.4192521
GACGCTC16500.017.4928742
TGCTCTT211550.016.9487725
TTCCGAT51600.016.1856529
AAACGGG73850.015.7937947
ACGGGAT46150.015.6009219
AACGGGA62200.014.9321138
GAAACGG76000.014.6838526
GCGTCGG17950.014.680111140-144
CTCTTCC266550.013.5866137
Produced by FastQC (version 0.11.5)