FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072150

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072150
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16822076
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA67450.062.0164269
TCTTCCG78350.053.3887448
CGTGTGC83150.051.9391671
CGACGCT7800.044.517381
GTGCTCT166850.025.5445214
GTGTGCT177500.024.7533972
TGTGCTC184600.023.7930153
GCTCTTC191200.022.480146
TCCGATC57200.019.056310-14
CCGATCT63350.018.82012210-14
CGCGATC25150.017.4639783
GCGCGAT24500.017.0796032
TTCCGAT63150.016.8733359
CGACGTC5652.8521754E-916.6447721
ACCGCGC48050.016.259731
GACGCTC19700.016.113962
TGCTCTT281800.015.6359055
GGCGCGA28750.014.8455781
CGTTGGA96200.014.2876721
CGATCTA19600.013.44416110-14
Produced by FastQC (version 0.11.5)