FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072156

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072156
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16771661
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA64150.061.5036859
TCTTCCG71150.055.4527248
CGTGTGC78350.050.8895651
CGACGCT6000.047.0359041
GTGCTCT165300.024.2594034
GTGTGCT176550.023.2585122
TGTGCTC184300.022.3474543
GCTCTTC188600.021.3014776
CCGATCT57250.019.24095710-14
TCCGATC54650.018.33833110-14
ACCGCGC44300.016.1713981
GCGCGAT24050.015.6036312
CGTTGGA91450.015.5091681
TTCCGAT58850.015.0478989
TGCTCTT273250.014.9923365
CGCGATC24700.014.8672473
GACGCTC17800.014.5955312
GGCGCGA29950.013.0470711
ACGGGAT63200.012.9868859
AAACGGG106200.012.9486977
Produced by FastQC (version 0.11.5)