FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072157

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072157
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12228094
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT77150.0137.098571
GACGCTC86000.0122.4380652
ACGCTCT92550.0113.772823
TTCCGAT94700.0111.3968359
CTTCCGA98700.0106.444838
CGCTCTT102850.0101.402144
TCTTCCG104300.0100.798677
GCTCTTC195450.054.2701955
CTCTTCC304150.034.89746
TCCGATC90950.024.47958210-14
CCGATCT94800.024.4266610-14
CGATCTA23300.019.5185510-14
CGTTGGA62400.018.652621
AACGGGA60400.015.9645538
ACGGGAT46200.015.887199
GAAACGG75450.015.2598286
CGTGTGC38150.015.0638861
AAACGGG74050.014.9652697
GGAAACG80600.014.1065765
CGATCTT54300.013.96777310-14
Produced by FastQC (version 0.11.5)