FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072158

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072158
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12228094
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA51850.075.070399
TCTTCCG55350.069.673468
CGTGTGC64400.060.311251
CGACGCT5250.049.782151
GTGCTCT127150.030.7313614
GTGTGCT135250.029.4283642
TGTGCTC137400.028.595933
GCTCTTC142950.027.586386
TCCGATC42800.020.97926710-14
CCGATCT48150.019.69421210-14
TGCTCTT207250.019.4795
TTCCGAT46700.016.91628310-14
CTCTTCC252500.016.1562987
CGTTGGA64700.015.5969951
GACGCTC14350.015.0469212
ACGGGAT45000.013.9099939
AACGGGA61050.013.3172018
AAACGGG73350.013.1439187
GAAACGG76250.012.929426
GCGTCGG15350.012.5006895140-144
Produced by FastQC (version 0.11.5)