FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072164

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072164
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15117797
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA63400.068.363949
TCTTCCG73250.058.8761028
CGTGTGC81750.055.7653471
CGACGCT5850.047.0044941
GTGCTCT159800.028.4283924
GTGTGCT169100.027.7800922
TGTGCTC176750.026.1533363
GCTCTTC180150.024.7386656
TCCGATC55000.020.13291210-14
CCGATCT58900.020.02218810-14
TGCTCTT257450.017.3387835
TTCCGAT60200.017.1024919
CGTTGGA79150.016.273491
CTCTTCC308350.014.7101287
CGACGTC5104.591053E-614.1886621
CGATCTA18150.013.96300910-14
ACGGGAT56400.013.6591769
AACGGGA74700.013.5899678
AAACGGG91450.012.9903277
GAAACGG94050.012.9374226
Produced by FastQC (version 0.11.5)