FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072174

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072174
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4779728
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA25950.015.2706711
ACGGGAT18700.014.2448069
GAAACGG30150.013.3720256
AACGGGA26000.013.29120358
AAACGGG30200.012.6347127
GGCCGAT4705.376618E-412.2637951
GGAAACG33150.011.5103575
CCCCGAT4450.004977632311.3336751
CGACATT4600.006371294610.9640991
CCCGATT6103.9940255E-410.6220682
CGATATT7251.7521477E-49.9379021
CCCAAAT79850.09.9254571
GACGATT7402.1704847E-49.7289222
CCCAAAG133200.09.6282771
CCACGTT12801.4817488E-89.5691141
CCCGAGT30100.09.3353561
CCCAAGT61850.09.2028031
TTCCGAT7150.00171404569.0621849
CCAAAGT123100.09.0065632
CCGAGTA22100.08.795652
Produced by FastQC (version 0.11.5)