FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072177

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072177
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4688804
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT3900.096.0677261
GACGCTC7500.048.897882
TTCCGAT8700.043.8078239
ACGCTCT9450.040.3297233
CTTCCGA10750.034.114448
CGCTCTT11550.032.374464
TCTTCCG12850.030.21817
CGTTGGA25300.019.0806471
TCCGATC5400.015.18259310-14
ACGGGAT17550.014.3412159
GAAACGG29900.014.1893326
AAACGGG29650.014.0662987
AACGGGA24550.013.4735758
CCCCGAT5158.21764E-512.5914021
GGAAACG32100.012.5448115
CCGATCT8000.011.1472210-14
AACGGGT7351.7353641E-510.76172358
ACGGGTT6406.2832475E-410.1124779
CCCAAAG135750.09.9783061
CGTATAA8709.972533E-69.9380421
Produced by FastQC (version 0.11.5)