FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11820701
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT11650.0101.8530041
GACGCTC18650.063.351192
TTCCGAT21250.058.9505589
CTTCCGA23950.051.7035688
ACGCTCT23900.050.320693
CGCTCTT29250.041.3629464
TCTTCCG30900.039.841467
CGTTGGA61150.019.7594991
ACGGGAT44350.016.0708629
AACGGGA58650.015.9577968
TCCGATC16700.015.69208610-14
AAACGGG74450.015.2788257
GAAACGG76400.014.4220226
GGAAACG79850.013.88909155
CCGATCT21600.012.73225710-14
GCTCTTC116150.010.7264245
CAAAGCG43500.09.6470531
CCCAAAG359750.09.6135331
AAGCGGC19300.09.3284773
CCGAGTA59600.09.1866482
Produced by FastQC (version 0.11.5)