FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12919649
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT22350.0120.758541
GACGCTC30950.087.523072
TTCCGAT36100.076.582029
ACGCTCT36550.074.280153
CTTCCGA41450.066.3501058
CGCTCTT43400.062.2243424
TCTTCCG46250.059.9310387
GCTCTTC131850.021.4103985
TCCGATC30350.019.35683310-14
CCGATCT33700.018.5435410-14
CGTTGGA68050.018.288871
AAACGGG77750.015.6490917
GAAACGG80950.015.3013736
ACGGGAT46900.015.1972559
AACGGGA65650.014.8047768
CGTCCGA3901.11243135E-414.7721666
GGAAACG82550.014.30695
CTCTTCC235950.012.6662166
AACGGGT19050.011.3378088
CCCGAAT29500.010.5470971
Produced by FastQC (version 0.11.5)