FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5156730
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT7500.0120.9950941
GACGCTC10750.084.2924042
ACGCTCT13900.066.224943
TTCCGAT13800.066.18379
CTTCCGA14650.062.343088
CGCTCTT16850.054.630664
TCTTCCG16850.053.3497357
CCGATCT11250.018.54028910-14
TCCGATC10400.018.25751110-14
GCTCTTC51700.018.0833635
CGTTGGA23950.015.9378171
ACGGGAT16550.012.1670819
AACGGGA25100.011.1741128
AAACGGG29250.011.0639147
GAAACGG31900.010.3703536
GGAAACG31600.010.2412235
CGATATT7853.5253273E-510.0920991
CTCTTCC99450.09.6900586
CGTGAAT8256.0826693E-59.6027861
CCACGGA11252.2684071E-79.6027851
Produced by FastQC (version 0.11.5)