FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4393215
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT6000.0118.862461
GACGCTC9050.077.871112
TTCCGAT10550.068.844279
CTTCCGA11350.064.6239248
ACGCTCT11750.060.589343
CGCTCTT12500.056.378694
TCTTCCG13600.053.4037137
TCCGATC8050.018.9403210-14
GCTCTTC40900.017.7581185
CCGATCT10150.015.87191410-14
CGTTGGA24550.015.5519611
ACGGGAT18550.014.7312159
AACGGGA23500.014.381998
AAACGGG29300.013.743887
GAAACGG29350.013.2307536
GGAAACG29450.012.2090995
CCCGAGT26350.010.6621491
CTCTTCC78000.010.0491846
CTCGCGA1150.00294588410.004749140-144
AACGGGT6750.00102421979.5879938
Produced by FastQC (version 0.11.5)