FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4393215
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA21500.016.092861
CGATATA4151.8708194E-413.8954421
AACGGGA21550.013.6973918
TTCCGAT6553.310979E-713.189899
ACGGGAT15650.012.4208319
AAACGGG27400.012.0867217
TCCGATC4201.15813236E-711.9991369
GAAACGG27750.011.9342776
CCGAGTA18250.011.8347652
GGAAACG28850.011.7287925
CCCGAGT24400.011.52141
CGCGATC5250.00136829410.9706393
CCCGAAT10104.6324203E-810.70533851
GCGCGAT5500.002016208410.4719732
CTTCCGA7853.539073E-510.0884468
CGAGTAG19650.09.1596463
GATTTCG12601.1997327E-79.14219955
CCAGCGA8155.6394556E-48.8444911
CCCGGGT18952.5465852E-118.7488121
CCGGGTT17303.4924597E-108.7392562
Produced by FastQC (version 0.11.5)