FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4384922
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA25900.020.0385721
AACGGGA24050.015.5663628
AAACGGG30600.014.1165547
GGAAACG32700.014.0906535
ACGGGAT17100.013.8936629
GAAACGG31900.013.3155856
CCGAGTA20950.012.027712
CGGCTTA4250.003518671711.8725271
CGAGTAG21700.011.6120053
ATATCCG4400.00460155911.453665
CCCGAGT29050.011.4142071
GCGTCGG7200.010.801283140-144
GTTGGAA61550.010.7611492
ACGGGAA15001.8189894E-1210.5591839
CCCGAAG7301.8690167E-49.8744111
CCCAAAG124950.09.4611011
CCCAAGT53400.09.3141211
CGACGAA1250.00580281079.216043130-134
GTAGCTG83000.09.0209886
GAGTAGC53100.08.948464
Produced by FastQC (version 0.11.5)