FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072197

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072197
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4115869
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT6500.0118.596791
GACGCTC9600.081.6428452
TTCCGAT11400.070.648419
ACGCTCT11450.068.451643
CTTCCGA12000.066.514318
TCTTCCG14050.056.8093837
CGCTCTT14050.054.7608644
CGTTGGA21800.020.48981
GCTCTTC41250.019.6977835
TCCGATC9300.018.86913510-14
CCGATCT10850.016.17354610-14
AAACGGG26400.014.4359497
AACGGGA20400.014.0994848
GGAAACG27550.013.8330235
GAAACGG27150.013.50713356
ACGGGAT15600.013.3678839
AACGGGT6454.060197E-612.2632718
CTCTTCC74350.011.3153436
CGCCAGT4700.00747889710.7300741
CCCAAAG123800.010.3586191
Produced by FastQC (version 0.11.5)