FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072198

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072198
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4115869
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA20850.018.3227921
TTCCGAT5752.0372681E-1017.529529
ACGGGAT13250.015.7576679
CTTCCGA6701.7807906E-915.043998
GAAACGG24850.014.1964426
GGAAACG25500.014.116915
AAACGGG25350.013.9164327
AACGGGA21000.013.7135718
TCTTCCG8453.3669494E-912.7803977
CCCAAAG118300.010.1754491
CCGAGTA18650.09.650972
GCGATCG1400.00141069789.258234140-144
CCAAAGT110100.09.1548362
CGAGTAG19303.6379788E-128.95293
CCCGAGT24800.08.7194981
CCCTATT28450.08.6142711
TCGTTGG16851.9608706E-98.54554759
GATTTCG13603.8560756E-78.4701465
GTTGGAA56000.08.3567072
ACGGGAA13804.8151014E-78.347399
Produced by FastQC (version 0.11.5)