FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072199

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072199
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10219879
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT16300.0118.970121
GACGCTC22750.085.823372
TTCCGAT25300.080.531719
CTTCCGA30350.066.8946468
ACGCTCT29800.066.217923
CGCTCTT32900.059.3218464
TCTTCCG34700.058.71627
TCCGATC20950.020.4815710-14
GCTCTTC105850.019.1186265
CGTTGGA53100.018.8050841
CCGATCT25500.017.61754610-14
ACGGGAT36300.016.4616159
AACGGGA51250.015.3120598
AAACGGG60400.015.1379747
GAAACGG60900.014.5455136
GGAAACG64100.013.0329045
CGAAACG4604.5032846E-412.5248765
CTCTTCC194550.010.9942716
CCGAGTA49950.010.3851952
CGAGTAG49200.010.2464683
Produced by FastQC (version 0.11.5)