FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072201

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072201
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5132954
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT10000.0123.9195861
GACGCTC12600.098.738552
TTCCGAT16050.082.443039
ACGCTCT15650.079.4955753
CTTCCGA17700.074.34928
CGCTCTT18900.066.206194
TCTTCCG20800.063.614047
GCTCTTC55200.024.2317645
TCCGATC14050.019.96447210-14
CCGATCT14850.019.66387610-14
ACGGGAT16050.016.1301579
CGTTGGA24700.015.459321
AACGGGA22500.014.3823048
CTCTTCC98900.013.8882736
AAACGGG28350.013.1901217
CGTCAAA6704.3222462E-712.9038091
GGAAACG30400.012.7741315
GAAACGG29900.012.2662036
GCGCGAA850.003582850811.844227135-139
CGATCTA4150.011.43840310-14
Produced by FastQC (version 0.11.5)