FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072204

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072204
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13067315
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA50850.075.185459
TCTTCCG56750.067.115848
CGTGTGC64000.060.3696751
CGACGCT4550.050.695871
GTGCTCT130800.029.5047684
GTGTGCT136650.028.935162
GCTCTTC142900.026.956086
TGTGCTC144500.026.6576043
TCCGATC39550.022.68294310-14
CCGATCT43950.020.7397110-14
TGCTCTT208750.018.9701775
CGTTGGA67450.018.8089581
GACGCTC13900.017.0986672
TTCCGAT47100.016.60109710-14
CTCTTCC251200.015.793097
ACGGGAT47150.015.4230369
GAAACGG80600.015.1861976
AAACGGG81750.015.1487187
AACGGGA65250.014.7862998
GGAAACG85000.013.891855
Produced by FastQC (version 0.11.5)