FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3776223
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA18300.011.0250471
TTCCGAT6003.427655E-410.7991819
AAACGGG22450.09.94133957
CACGTTT11302.4314977E-79.5567972
GAAACGG23300.09.2696836
AACGGGA17957.2759576E-129.2249278
GCGTCGG8000.09.000628140-144
GATTTCG12851.6214835E-78.96435
CCCGAGT22750.08.8684991
TCGTTGG16401.1787051E-98.7798219
CCACGTT11252.2471355E-58.3265351
CCGAGTA17403.5815901E-98.2752352
CCCAAAG115550.08.2314721
TTCGTTG19353.6379788E-108.1854268
CCGGGTT15102.1735832E-78.1053462
CGAGTAG17906.0772436E-98.0440843
ATTTCGT14501.0195308E-67.9442256
TTTCGTT20107.894414E-107.8799997
CCCAATA24601.4551915E-117.6157331
CCCAAGT46650.07.56864171
Produced by FastQC (version 0.11.5)