FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072208

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072208
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10048663
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA29000.064.551699
TCTTCCG31650.059.3749668
CGTGTGC42400.044.5412981
GTGCTCT92400.020.259934
GTGTGCT99300.019.8735852
TCCGATC20800.019.86925910-14
TGTGCTC100550.019.1190263
GCTCTTC105350.018.6579826
CCGATCT24550.017.36214410-14
TTCCGAT24950.015.29461110-14
CGTTGGA55850.014.0679481
TGCTCTT159400.012.6023775
GATTTCG34250.011.5621525
ACGGGAT38500.011.4077859
GGAAACG67600.011.3965575
CGAGTAT6354.5487675E-511.3515121
AAACGGG64850.011.21367557
GAAACGG66600.011.135246
AACGGGA52850.010.8988728
CTCTTCC186400.010.8541797
Produced by FastQC (version 0.11.5)