FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072209

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072209
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4242219
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT5250.0115.273791
GACGCTC8900.067.8751452
TTCCGAT9800.063.84029
ACGCTCT9900.061.745493
CTTCCGA10850.056.999338
CGCTCTT11650.053.7050174
TCTTCCG13150.047.0298657
CGTTGGA21650.017.9699331
GCTCTTC41500.016.2892575
TCCGATC8200.016.13854610-14
ACGGGAT14800.015.5485299
CCGATCT9300.014.53902610-14
AAACGGG25550.014.0727887
AACGGGA20600.013.6143998
GGAAACG26000.013.0000815
GAAACGG25750.012.5677286
CGCGACG955.9751316E-412.1112585135-139
AACGGGT5902.965456E-410.9696198
CGAGAAT6050.00441453339.5267591
CCCATAA33150.09.1280151
Produced by FastQC (version 0.11.5)