FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072212

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072212
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10538066
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA29450.063.8097089
TCTTCCG36250.052.0397388
CGTGTGC42050.046.1079181
GTGCTCT94050.021.2061984
GTGTGCT102950.019.8682482
TGTGCTC103200.019.3957653
TCCGATC22400.018.89972510-14
GCTCTTC108400.018.0003876
CCGATCT25250.017.22271710-14
CGTTGGA54850.016.1628421
TTCCGAT25800.014.790482510-14
AACGGGA50750.014.3293728
ACGGGAT38650.014.1577959
AAACGGG64200.013.4582337
GAAACGG67200.013.071716
GGAAACG67700.012.5497535
TGCTCTT164700.012.5467185
CTCTTCC190000.010.3833817
AACGGGT16852.0736479E-108.9734878
CGTTGAT14207.95153E-88.6287881
Produced by FastQC (version 0.11.5)