FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072215

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072215
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10138814
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT11350.0112.827291
GACGCTC18850.067.259992
TTCCGAT20050.065.352129
CTTCCGA24850.052.7287678
ACGCTCT24400.052.5308273
TCTTCCG28150.046.0359087
CGCTCTT29450.042.3004384
CGTTGGA53000.018.9747331
AACGGGA49350.017.0687358
TCCGATC15950.017.0621510-14
ACGGGAT37800.016.9512169
AAACGGG60300.016.4764797
GAAACGG62350.015.7067556
CCGATCT19150.015.63965510-14
GGAAACG64150.014.8170345
GCTCTTC96950.013.7406715
CCCGAAT20750.010.4601981
CCGAGTA51800.010.2909962
CCCAAGT133500.010.025991
CGAGTAG50700.09.9420073
Produced by FastQC (version 0.11.5)