FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072216

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072216
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10138814
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA25950.055.490759
TCTTCCG30900.046.8344658
CGTGTGC38500.040.4414521
CGACGCT3409.640644E-1125.441111
TCCGATC18050.017.94979310-14
GTGCTCT86750.017.6782024
GTGTGCT97850.017.0764922
CGTTGGA51550.016.9196131
CCGATCT21400.015.81277910-14
GCTCTTC99550.015.6944626
TGTGCTC99300.015.371443
ACGGGAT36000.014.399859
AACGGGA49300.014.0201378
AAACGGG59300.013.9627567
TTCCGAT23100.013.02832110-14
GAAACGG62000.013.0063176
GGAAACG62250.012.6070985
TGCTCTT150250.010.3506415
CCGAGTA48800.010.0359992
GCGTCGG8800.09.327313140-144
Produced by FastQC (version 0.11.5)