FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072217

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072217
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6380846
Sequences flagged as poor quality0
Sequence length150
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT7400.0106.099611
GACGCTC10900.071.914662
TTCCGAT14600.052.7066429
ACGCTCT15250.050.929723
CTTCCGA15350.050.129448
TCTTCCG18000.043.1487927
CGCTCTT19650.039.525614
CGTTGGA32900.018.6098141
GAAACGG37600.016.6398286
TCCGATC10050.015.60057310-14
ACGGGAT23150.015.5329319
AAACGGG38300.015.0213387
CCGATCT11850.014.93024210-14
AACGGGA31850.014.4506698
GGAAACG40200.014.1324835
GCTCTTC61100.013.3000915
AACGGGT11201.382432E-1011.5577138
CGCAAGC4850.00945532310.3962171
CCCCGAA9302.0201423E-610.0688391
CCGAGTA33600.010.0594912
Produced by FastQC (version 0.11.5)