FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072221

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072221
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3969875
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT12350.0131.844961
GACGCTC15050.0107.023562
TTCCGAT17350.094.9094249
ACGCTCT18000.089.883073
CTTCCGA18500.087.842488
CGCTCTT19450.082.073184
TCTTCCG19900.081.301277
GCTCTTC46350.035.52665
ACGCCGA900.003778592731.9584242
TCCGATC15200.022.1426310-14
CCGATCT15900.022.07240710-14
CTCTTCC82550.019.7731866
CGTTGGA19450.016.6692071
CGATCTA4250.013.87560410-14
ACGGGAT13350.013.4657989
AAACGGG23650.013.3779457
GAAACGG23600.012.7969126
AACGGGA19900.012.2855258
GGAAACG25550.012.1016745
AACGGGT5952.3973651E-512.0851198
Produced by FastQC (version 0.11.5)