FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072223

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072223
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10049124
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT31700.0135.127371
GACGCTC39500.0108.3438642
TTCCGAT43600.0100.726769
ACGCTCT43200.099.18923
CTTCCGA45700.095.625568
TCTTCCG50500.086.2512747
CGCTCTT49700.085.637164
GCTCTTC123100.035.627925
TCCGATC39600.022.50737410-14
CCGATCT42150.022.1705510-14
CTCTTCC207000.021.5701186
CGTTGGA51000.014.7551621
CGATCTA13250.013.475210-14
AAACGGG60400.012.7540577
ACGGGAT34650.012.4666439
AACGGGA50700.011.7861418
GAAACGG64250.011.7692126
AACGGGT17100.010.9465888
GGAAACG65750.010.7339985
CGATCTT31750.010.56675310-14
Produced by FastQC (version 0.11.5)