FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072224

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072224
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10049124
Sequences flagged as poor quality0
Sequence length150
%GC40

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA52100.094.109939
TCTTCCG56800.086.070868
CGTGTGC61450.080.831621
GTGCTCT111200.044.7412384
GTGTGCT119450.041.9683652
GCTCTTC121900.040.9911886
TGTGCTC122050.040.9408043
CGACGCT3500.037.0756231
TGCTCTT177500.028.5567655
CTCTTCC211800.023.9321357
TCCGATC45650.023.59464510-14
CCGATCT47800.022.77437610-14
TTCCGAT48850.020.30987710-14
CGTTGGA53650.015.31861
ACGGGAT37150.014.5354519
CGATCTA11950.014.21892310-14
GAAACGG62250.013.8795896
AAACGGG60950.013.8212377
GACGCTC9155.638867E-1113.3821782
AACGGGA52300.013.3537978
Produced by FastQC (version 0.11.5)