FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072225

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072225
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4558797
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT8800.0125.24031
GACGCTC13550.081.7266162
TTCCGAT13650.080.075169
ACGCTCT15300.072.848793
CTTCCGA16050.068.547848
CGCTCTT17850.061.6361244
TCTTCCG18150.060.6166847
CGTTGGA24650.022.2091561
GCTCTTC49850.022.0703075
TCCGATC11550.019.42727710-14
AACGGGA23150.017.7052158
CCGATCT13750.017.6788210-14
AAACGGG28250.017.0542937
GAAACGG30000.017.018426
GGAAACG30800.016.1094425
ACGGGAT16900.015.3180219
CTCTTCC91700.012.7036296
AAGCGGC7752.5651461E-611.1342673
AACGGGT6606.9055386E-510.8951558
CCCCGAA7552.2900282E-510.4949681
Produced by FastQC (version 0.11.5)