FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072228

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072228
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11758934
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA41700.073.0363169
TCTTCCG46850.065.622798
CGTGTGC59600.053.2141271
GTGCTCT118050.027.0193044
GTGTGCT125000.025.6402972
TGTGCTC130450.024.8373243
GCTCTTC129800.024.0741756
CGTTGGA66350.021.1843031
TCCGATC31750.020.99915310-14
CCGATCT36450.018.72601510-14
TGCTCTT189950.016.943565
TTCCGAT36650.016.89503110-14
AACGGGA63550.016.7680518
AAACGGG78650.016.4782147
ACGGGAT46300.016.0173539
GAAACGG80050.015.9201946
GGAAACG79700.015.8094275
CTCTTCC239100.013.5509387
CGATCTA12000.010.92005210-14
AACGGGT19750.09.8431268
Produced by FastQC (version 0.11.5)