FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072229

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072229
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4237838
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT6650.0123.491621
GACGCTC10700.077.292072
TTCCGAT11050.076.1446469
CTTCCGA13000.065.827778
ACGCTCT12950.063.862953
CGCTCTT14200.057.2283174
TCTTCCG15300.056.4021037
ACGGGAT15300.022.0913479
CGTTGGA22700.021.2619571
AACGGGA21050.021.1809358
AAACGGG25750.019.549087
GCTCTTC45200.019.092545
GAAACGG26200.018.9395086
TCCGATC9600.018.12901710-14
GGAAACG27150.017.4821575
CCGATCT11050.016.79142610-14
CGTATAA8053.1414675E-711.6332691
AAGCGGC8103.4792174E-711.5419523
CAAAGCG15000.011.5258851
CTCTTCC85800.010.7286146
Produced by FastQC (version 0.11.5)