FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072230

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072230
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4237838
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA23150.021.7884331
ACGGGAT15750.016.4558969
AACGGGA23600.014.6429598
AAACGGG28400.013.9426057
GAAACGG29250.013.0451656
TTCCGAT5301.0824549E-412.2254899
CGTTAAT5551.6497767E-411.6849941
GGAAACG32100.011.6626685
GTTGGAA59150.010.1023622
AAGCGGC8508.463332E-59.316943
CCCAAAG117700.09.1831921
ATCTCCG6300.0061980939.1421647
CTTCCGA7954.4545188E-49.0559178
CCGAGTA20401.8189894E-128.8228612
TCGTTGG19605.2750693E-118.4483399
CCCCAAT23050.08.440571
AGCGGCT10307.360964E-58.3877144
TTCGTTG22009.094947E-128.1811978
CGTGAAG10601.0184347E-48.1574491
CGGCTGA10601.02884645E-48.1503266
Produced by FastQC (version 0.11.5)