FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072231

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072231
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11288380
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT21650.0122.326631
GACGCTC29900.088.187992
TTCCGAT33250.080.331429
ACGCTCT35700.074.035753
CTTCCGA37000.071.800558
CGCTCTT39800.066.2284
TCTTCCG40950.064.523117
CGTTGGA59750.023.3731461
GCTCTTC122550.021.7436315
TCCGATC28500.019.45131710-14
CCGATCT30100.019.18275610-14
ACGGGAT43250.019.1432179
AAACGGG73450.018.6236557
AACGGGA60450.018.579398
GAAACGG73850.018.3337356
GGAAACG76450.017.427615
TCGTTCG3000.0049189114.4036854
AACGGGT17950.013.2358748
CTCTTCC232450.011.990166
GTTGGAA153300.010.7150012
Produced by FastQC (version 0.11.5)