FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072234

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072234
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4928229
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA26450.019.8984261
ACGGGAT18700.017.3247249
AACGGGA26450.016.059128
AAACGGG31250.015.8962427
GGAAACG33300.015.13384155
GAAACGG31750.014.9656516
CGTCATT9157.9853635E-1012.6072521
CCGAGTA24050.011.6746792
CGAGTAG24300.011.2582983
CGTAGAA7351.9981306E-49.8092141
CCCGAGT34050.09.7400751
GTTGGAA68800.09.2085162
GACGATT6650.0096562368.6609142
CCCAAGT65100.08.5276881
CGCAAAA8050.00494737468.0606151
CGCGTCG3701.121407E-87.784304140-144
CAAAGCG20451.0895747E-97.75623461
ACGATTC9300.0021092197.74127443
GTAGCTG97550.07.6754086
CCGCTGT10357.1068725E-47.65152032
Produced by FastQC (version 0.11.5)