FastQCFastQC Report
Sat 23 Apr 2022
EGAF00005072239

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005072239
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13503646
Sequences flagged as poor quality0
Sequence length150
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGCT11050.095.576351
GACGCTC21550.049.5071222
TTCCGAT24550.043.3988089
ACGCTCT28150.038.397413
CTTCCGA29450.035.9335068
TCTTCCG35950.029.8375367
CGCTCTT36500.028.7965454
CGTTGGA72150.018.9489571
CGACGAA2359.3485694E-418.4689941
ACGGGAT50150.016.2209039
AACGGGA67000.015.6872058
GAAACGG84600.015.31714256
AAACGGG83550.015.07881457
GGAAACG90500.014.0005315
TCCGATC19750.011.22667510-14
AACGGGT19950.010.8254498
CCGATCT23450.010.62186610-14
GCTCTTC128150.08.8198565
CCCAAAG424300.08.5754091
ACCGCGC34050.08.4977261
Produced by FastQC (version 0.11.5)